Professor Em. Ioannis P. Gerothanassis PhD, DSc Professor Adjunct, ICCBS, University of Karachi, Pakistan
Nuclear Magnetic Resonance (NMR) Spectroscopy is based on nuclear magnetic moment transition within a strong static and homogenous magnetic field B0. These transitions as caused by the application of an electromagnetic irradiation in the region of 1000 – 10 MHz depending on the field strength. The resonance frequency of a particular nucleus, such as 1H, is not constant but it depends on the electronic environment of the proton. Based on this property NMR has made a tremendous impact, at a molecular level, in many areas of chemistry, biology and medicine.
SCIENTIFIC FIELDS
ΕΛΙΔΕΚ 2019-2023
Project Title
Investigation of interactions of unsaturated fatty acids with albumin and the anti-apoptotic protein Bcl-2 with emphasis in the use of NMR methodologies at a cellular level (in-cell NMR)
Principal Investigator
Ioannis P. Gerothanassis
Host Institution
University of Ioannina
WP Number: 1
WP Objectives: Fast screening of bioactive unsaturated lipids interactions with bovine/human serum albumin and the anti-apoptotic Bcl-2 proteins in vitro.
WP Number: 2
Objectives - Description of Work: Study of structural and conformational properties of lipid interactions with the target protein, Bcl-2, at the cellular level using in-cell NMR.
WP Number: 3
Objectives - Description of Work: Investigation of in vitro cytotoxicity in cancer cell lines. Tasks: Cytotoxic activity of FFAs will be tested against: (i) Human T-cell leukemia Jurkat Bcl-2 cell line overexpressing Bcl-2 and (ii) the corresponding Jurkat Puro control cell line.
Recent Articles
Test Article
This is a test article title to see how this page is work The ship was making her way steadily through small waves which slapped her and then fizzled like effervescing water, leaving a little border of bubbles and foam on either side. The colourless October sky above was thinly clouded as if by the […]
